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l lg ythdf1 proteintech  (Proteintech)


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    Structured Review

    Proteintech l lg ythdf1 proteintech
    L Lg Ythdf1 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf1+proteintech/YTHDF1+Antibody/pm41520337-278-73-75
    Average 96 stars, based on 411 article reviews
    l lg ythdf1 proteintech - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: YTHDF1 Facilitates the Progression of Hepatocellular Carcinoma by Promoting FZD5 mRNA Translation in an m6A-Dependent Manner
    Article Snippet: .. YTHDF1 Proteintech (17479-1-AP) WB 1:1000 IHC 1:100 RIP 2.0 μg FZD5 Abcam (ab75234) WB 1:500 IHC 4.0 μg c-MYC Proteintech (10828-1-AP) WB 1:2000 ChIP 2.0 μg Abcam (ab32072) IHC 1:100 USF1 SANTA CRUZ (sc-390027) IHC 1:50 ChIP 2.0 β-catenin Proteintech (51067-2-AP) WB 1:2000 IHC 1:1000 GAPDH Proteintech (60004-1-Ig) WB 1:5000 β-actin Proteintech (60008-1-Ig) WB 1:5000 HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) Proteintech (SA00001-1) WB 1:10000 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) Proteintech (SA00001-2) WB 1:10000 ..

    Article Title: m 6 A Modification of Profilin-1 in Vascular Smooth Muscle Cells Drives Phenotype Switching and Neointimal Hyperplasia via Activation of the p-ANXA2/STAT3 Pathway
    Article Snippet: Species Vendor or Source Background Strain Persistent ID / URL Parent - Male Mouse Cyagen Bioscience Inc. PFN1F/F https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-CKO-04251 Parent - Female Mouse Cyagen Bioscience Inc. PFN1F/F https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-CKO-04251 Parent - Male Mouse Cyagen Bioscience Inc. YTHDF3-/- https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-KO-06231 Parent - Female Mouse Cyagen Bioscience Inc. YTHDF3-/- https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-KO-06231 Parent - Male Mouse Cyagen Bioscience Inc. MYH11CreERT2 Custom-made Parent - Male Mouse Cyagen Bioscience Inc. Tagln-Cre Custom-made Parent - Male Mouse Shanghai Model Organisms Center METTL3F/F https://www.modelorg.com/portal/article/index/id /3721/post_type/3.html Parent - Female Mouse Shanghai Model Organisms Center METTL3F/F https://www.modelorg.com/portal/article/index/id /3721/post_type/3.html .. Target antigen Vendor or Source Catalog # Working concentration PFN1 Abcam ab124904 WB: 0.1ug/ml, IHC: 2 ug/ml, IF: 10 ug/ml PFN1 Santa cruz sc137235 IP: 500 ug/ml GAPDH Affinity AF7021 0.1ug/ml α-SMA Abcam ab7817 WB: 1ug/ml, IHC: 0.5 ug/ml, IF: 2 ug/ml β-actin Affinity AF7018 0.1ug/ml SM22α Proteintech 10493 WB: 0.075 ug/ml IF: 3.8 ug/ml SMMHC Proteintech 21404 WB: 0.7 ug/ml, IF: 7 ug/ml Vimentin CST 5741 WB: 1 ug/ml, IF: 20 ug/ml S100A4 Proteintech 66489 WB: 0.17 ug/ml, IF: 17 ug/ml METTL3 Abcam ab195352 WB: 1 ug/ml, IHC: 2 ug/ml, IP: 20 ug/ml ALKBH5 Abcam ab195377 1 ug/ml Hitone-H3 Proteintech 17168 0.05 ug/ml YTHDF1 Proteintech 17479 0.33 ug/ml YTHDF3 Proteintech, Santa cruz 25537 WB: 0.085 ug/ml YTHDF3 Santa cruz sc377119 IP: 200 ug/ml GST-Tag CST 2625 1 ug/ml Flag-Tag CST 8146 1 ug/ml Myc-Tag CST 2276 1 ug/ml t-ANXA2 CST 8235 WB: 1 ug/ml, IF: 10 ug/ml p-ANXA2 (Tyr23) Affinity AF7096 WB: 1 ug/ml, IHC: 10 ug/ml LaminA/C CST 4777 0.5 ug/ml Src CST 2110 1 ug/ml pp60-Src CST 6943 1 ug/ml p-Tyr Santa cruz sc508 IP: 500 ug/ml ..

    Immunohistochemistry:

    Article Title: YTHDF1 Facilitates the Progression of Hepatocellular Carcinoma by Promoting FZD5 mRNA Translation in an m6A-Dependent Manner
    Article Snippet: .. YTHDF1 Proteintech (17479-1-AP) WB 1:1000 IHC 1:100 RIP 2.0 μg FZD5 Abcam (ab75234) WB 1:500 IHC 4.0 μg c-MYC Proteintech (10828-1-AP) WB 1:2000 ChIP 2.0 μg Abcam (ab32072) IHC 1:100 USF1 SANTA CRUZ (sc-390027) IHC 1:50 ChIP 2.0 β-catenin Proteintech (51067-2-AP) WB 1:2000 IHC 1:1000 GAPDH Proteintech (60004-1-Ig) WB 1:5000 β-actin Proteintech (60008-1-Ig) WB 1:5000 HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) Proteintech (SA00001-1) WB 1:10000 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) Proteintech (SA00001-2) WB 1:10000 ..

    Article Title: m 6 A Modification of Profilin-1 in Vascular Smooth Muscle Cells Drives Phenotype Switching and Neointimal Hyperplasia via Activation of the p-ANXA2/STAT3 Pathway
    Article Snippet: Species Vendor or Source Background Strain Persistent ID / URL Parent - Male Mouse Cyagen Bioscience Inc. PFN1F/F https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-CKO-04251 Parent - Female Mouse Cyagen Bioscience Inc. PFN1F/F https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-CKO-04251 Parent - Male Mouse Cyagen Bioscience Inc. YTHDF3-/- https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-KO-06231 Parent - Female Mouse Cyagen Bioscience Inc. YTHDF3-/- https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-KO-06231 Parent - Male Mouse Cyagen Bioscience Inc. MYH11CreERT2 Custom-made Parent - Male Mouse Cyagen Bioscience Inc. Tagln-Cre Custom-made Parent - Male Mouse Shanghai Model Organisms Center METTL3F/F https://www.modelorg.com/portal/article/index/id /3721/post_type/3.html Parent - Female Mouse Shanghai Model Organisms Center METTL3F/F https://www.modelorg.com/portal/article/index/id /3721/post_type/3.html .. Target antigen Vendor or Source Catalog # Working concentration PFN1 Abcam ab124904 WB: 0.1ug/ml, IHC: 2 ug/ml, IF: 10 ug/ml PFN1 Santa cruz sc137235 IP: 500 ug/ml GAPDH Affinity AF7021 0.1ug/ml α-SMA Abcam ab7817 WB: 1ug/ml, IHC: 0.5 ug/ml, IF: 2 ug/ml β-actin Affinity AF7018 0.1ug/ml SM22α Proteintech 10493 WB: 0.075 ug/ml IF: 3.8 ug/ml SMMHC Proteintech 21404 WB: 0.7 ug/ml, IF: 7 ug/ml Vimentin CST 5741 WB: 1 ug/ml, IF: 20 ug/ml S100A4 Proteintech 66489 WB: 0.17 ug/ml, IF: 17 ug/ml METTL3 Abcam ab195352 WB: 1 ug/ml, IHC: 2 ug/ml, IP: 20 ug/ml ALKBH5 Abcam ab195377 1 ug/ml Hitone-H3 Proteintech 17168 0.05 ug/ml YTHDF1 Proteintech 17479 0.33 ug/ml YTHDF3 Proteintech, Santa cruz 25537 WB: 0.085 ug/ml YTHDF3 Santa cruz sc377119 IP: 200 ug/ml GST-Tag CST 2625 1 ug/ml Flag-Tag CST 8146 1 ug/ml Myc-Tag CST 2276 1 ug/ml t-ANXA2 CST 8235 WB: 1 ug/ml, IF: 10 ug/ml p-ANXA2 (Tyr23) Affinity AF7096 WB: 1 ug/ml, IHC: 10 ug/ml LaminA/C CST 4777 0.5 ug/ml Src CST 2110 1 ug/ml pp60-Src CST 6943 1 ug/ml p-Tyr Santa cruz sc508 IP: 500 ug/ml ..

    Chromatin Immunoprecipitation:

    Article Title: YTHDF1 Facilitates the Progression of Hepatocellular Carcinoma by Promoting FZD5 mRNA Translation in an m6A-Dependent Manner
    Article Snippet: .. YTHDF1 Proteintech (17479-1-AP) WB 1:1000 IHC 1:100 RIP 2.0 μg FZD5 Abcam (ab75234) WB 1:500 IHC 4.0 μg c-MYC Proteintech (10828-1-AP) WB 1:2000 ChIP 2.0 μg Abcam (ab32072) IHC 1:100 USF1 SANTA CRUZ (sc-390027) IHC 1:50 ChIP 2.0 β-catenin Proteintech (51067-2-AP) WB 1:2000 IHC 1:1000 GAPDH Proteintech (60004-1-Ig) WB 1:5000 β-actin Proteintech (60008-1-Ig) WB 1:5000 HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) Proteintech (SA00001-1) WB 1:10000 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) Proteintech (SA00001-2) WB 1:10000 ..

    Concentration Assay:

    Article Title: m 6 A Modification of Profilin-1 in Vascular Smooth Muscle Cells Drives Phenotype Switching and Neointimal Hyperplasia via Activation of the p-ANXA2/STAT3 Pathway
    Article Snippet: Species Vendor or Source Background Strain Persistent ID / URL Parent - Male Mouse Cyagen Bioscience Inc. PFN1F/F https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-CKO-04251 Parent - Female Mouse Cyagen Bioscience Inc. PFN1F/F https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-CKO-04251 Parent - Male Mouse Cyagen Bioscience Inc. YTHDF3-/- https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-KO-06231 Parent - Female Mouse Cyagen Bioscience Inc. YTHDF3-/- https://www.cyagen.com/cn/zh-cn/sperm-bankcn/S-KO-06231 Parent - Male Mouse Cyagen Bioscience Inc. MYH11CreERT2 Custom-made Parent - Male Mouse Cyagen Bioscience Inc. Tagln-Cre Custom-made Parent - Male Mouse Shanghai Model Organisms Center METTL3F/F https://www.modelorg.com/portal/article/index/id /3721/post_type/3.html Parent - Female Mouse Shanghai Model Organisms Center METTL3F/F https://www.modelorg.com/portal/article/index/id /3721/post_type/3.html .. Target antigen Vendor or Source Catalog # Working concentration PFN1 Abcam ab124904 WB: 0.1ug/ml, IHC: 2 ug/ml, IF: 10 ug/ml PFN1 Santa cruz sc137235 IP: 500 ug/ml GAPDH Affinity AF7021 0.1ug/ml α-SMA Abcam ab7817 WB: 1ug/ml, IHC: 0.5 ug/ml, IF: 2 ug/ml β-actin Affinity AF7018 0.1ug/ml SM22α Proteintech 10493 WB: 0.075 ug/ml IF: 3.8 ug/ml SMMHC Proteintech 21404 WB: 0.7 ug/ml, IF: 7 ug/ml Vimentin CST 5741 WB: 1 ug/ml, IF: 20 ug/ml S100A4 Proteintech 66489 WB: 0.17 ug/ml, IF: 17 ug/ml METTL3 Abcam ab195352 WB: 1 ug/ml, IHC: 2 ug/ml, IP: 20 ug/ml ALKBH5 Abcam ab195377 1 ug/ml Hitone-H3 Proteintech 17168 0.05 ug/ml YTHDF1 Proteintech 17479 0.33 ug/ml YTHDF3 Proteintech, Santa cruz 25537 WB: 0.085 ug/ml YTHDF3 Santa cruz sc377119 IP: 200 ug/ml GST-Tag CST 2625 1 ug/ml Flag-Tag CST 8146 1 ug/ml Myc-Tag CST 2276 1 ug/ml t-ANXA2 CST 8235 WB: 1 ug/ml, IF: 10 ug/ml p-ANXA2 (Tyr23) Affinity AF7096 WB: 1 ug/ml, IHC: 10 ug/ml LaminA/C CST 4777 0.5 ug/ml Src CST 2110 1 ug/ml pp60-Src CST 6943 1 ug/ml p-Tyr Santa cruz sc508 IP: 500 ug/ml ..



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    Proteintech resource source identifier antibodies anti ythdf1 proteintech
    Figure 5. The <t>m6A/YTHDF1/APC</t> pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.
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    Proteintech ythdf1 proteintech 17479 1 ap ythdf2 proteintech 24744 1 ap wtap proteintech 60188 1 ig gapdh proteintech 10494 1 ap bacterial
    Figure 5. The <t>m6A/YTHDF1/APC</t> pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.
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    Image Search Results


    Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.

    Journal: Cell reports

    Article Title: m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development.

    doi: 10.1016/j.celrep.2025.115727

    Figure Lengend Snippet: Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-YTHDF1 Proteintech Cat#:17479-1-AP; RRID:AB_2217473 Anti -YTHDF2 Proteintech Cat#:24744-1-AP; RRID:AB_2687435 Anti-YTHDF3 Santa Cruz Biotech Cat#:Sc-377119; RRID:AB_2687436 Anti-METTL14 ATLAS ANTIBODIES (Sigma) Cat#:HPA038002; RRID:AB_10672401 Anti-Tubulin (Tyrosinated) Abcam Cat#:ab6160; RRID:AB_305328 Anti-APC Millipore Cat#:OP80; RRID:AB_2057371 Anti-APC-C (C-20) Santa Cruz Cat#:SC-896; RRID:AB_2057493 Anti-FMRP Abcam Cat#:ab17722; RRID:AB_2278530 Anti-HA-tag MBL Cat#:M180-3; RRID:AB_10951811 Anti-HA tag Abcam Cat#:ab9110; RRID:AB_307019 Anti-GAPDH (14C10) Cell Signaling Cat#:2118; RRID:AB_561053 Anti-Puromycin Millipore Cat#:MABE343; RRID:AB_2566826 Anti-DCX Abcam Cat#:ab18723; RRID:AB_732011 Anti-CRMP2 Biolegend Cat#:869001; RRID:AB_732011 Anti-GFP Abcam Cat#:ab13970; RRID:AB_300798 Anti-Tau Abcam Cat#:ab75714; RRID:AB_1310734 Anti-MAP2 Abcam Cat#:ab5392; RRID:AB_2138153 Anti-Beta-actin Cayman Chemicals Cat#:32127; RRID:AB_2810170 Anti-Cre Recombinase Millipore Cat#:MAB3120; RRID:AB_2085748 Anti-Tubb2b Abcepta Cat#:AP11940a; RRID:AB_10820514 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 488 Thermofisher Cat#:A11001; RRID:AB_2534069 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 568 Thermofisher Cat#:A11004; RRID:AB_2534072 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21050; RRID:AB_2535718 Goat Anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21070; RRID:AB_2535731 Donkey anti-Chicken IgY (H + L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluo 568 Thermofisher Cat# A78950; RRID:AB_2921072 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#:C3040H Chemicals, peptides, and recombinant proteins Puromycin InvivoGen Cat#:58-58-2 ProLong Diamond antifade mountant Thermofisher Cat#:P36965 DPBS Thermofisher Cat#:10010023 Neurobasal medium Thermofisher Cat#:10888022 MEM Thermofisher Cat#:11095080 Penicillin/Streptomycin Thermofisher Cat#:15140122 (Continued on next page) 18 Cell Reports 44, 115727, June 24, 2025

    Techniques: Cell Culture, In Vivo, Expressing

    Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation

    Journal: Cell reports

    Article Title: m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development.

    doi: 10.1016/j.celrep.2025.115727

    Figure Lengend Snippet: Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-YTHDF1 Proteintech Cat#:17479-1-AP; RRID:AB_2217473 Anti -YTHDF2 Proteintech Cat#:24744-1-AP; RRID:AB_2687435 Anti-YTHDF3 Santa Cruz Biotech Cat#:Sc-377119; RRID:AB_2687436 Anti-METTL14 ATLAS ANTIBODIES (Sigma) Cat#:HPA038002; RRID:AB_10672401 Anti-Tubulin (Tyrosinated) Abcam Cat#:ab6160; RRID:AB_305328 Anti-APC Millipore Cat#:OP80; RRID:AB_2057371 Anti-APC-C (C-20) Santa Cruz Cat#:SC-896; RRID:AB_2057493 Anti-FMRP Abcam Cat#:ab17722; RRID:AB_2278530 Anti-HA-tag MBL Cat#:M180-3; RRID:AB_10951811 Anti-HA tag Abcam Cat#:ab9110; RRID:AB_307019 Anti-GAPDH (14C10) Cell Signaling Cat#:2118; RRID:AB_561053 Anti-Puromycin Millipore Cat#:MABE343; RRID:AB_2566826 Anti-DCX Abcam Cat#:ab18723; RRID:AB_732011 Anti-CRMP2 Biolegend Cat#:869001; RRID:AB_732011 Anti-GFP Abcam Cat#:ab13970; RRID:AB_300798 Anti-Tau Abcam Cat#:ab75714; RRID:AB_1310734 Anti-MAP2 Abcam Cat#:ab5392; RRID:AB_2138153 Anti-Beta-actin Cayman Chemicals Cat#:32127; RRID:AB_2810170 Anti-Cre Recombinase Millipore Cat#:MAB3120; RRID:AB_2085748 Anti-Tubb2b Abcepta Cat#:AP11940a; RRID:AB_10820514 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 488 Thermofisher Cat#:A11001; RRID:AB_2534069 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 568 Thermofisher Cat#:A11004; RRID:AB_2534072 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21050; RRID:AB_2535718 Goat Anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21070; RRID:AB_2535731 Donkey anti-Chicken IgY (H + L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluo 568 Thermofisher Cat# A78950; RRID:AB_2921072 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#:C3040H Chemicals, peptides, and recombinant proteins Puromycin InvivoGen Cat#:58-58-2 ProLong Diamond antifade mountant Thermofisher Cat#:P36965 DPBS Thermofisher Cat#:10010023 Neurobasal medium Thermofisher Cat#:10888022 MEM Thermofisher Cat#:11095080 Penicillin/Streptomycin Thermofisher Cat#:15140122 (Continued on next page) 18 Cell Reports 44, 115727, June 24, 2025

    Techniques: